Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VII. Isolation and sequences of three large cyanogen bromide peptides.
نویسندگان
چکیده
The isolation and sequences of three peptides of large size from a cyanogen bromide digest of the NAD-specific glutamate dehydrogenase of Neurospora crassa are reported. These three peptides comprise 86, 117, and 134 residues, respectively, and represent approximately 30% of the estimated 1030 residues in the peptide chain.
منابع مشابه
Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VI. Isolation and sequences of eighteen fragments from the cyanogen bromide digest.
The 1030-residue polypeptide chain of the NAD-specific glutamate dehydrogenase of Neurospora crassa was fragmented by treatment with cyanogen bromide. The isolation and sequences of 18 fragments ranging in size from 4 to 51 residues are described. Some of these peptides proved to be cleavage products resulting from hydrolysis at acid-sensitive aspartyl-prolyl bonds. Some overlaps could be deduc...
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Neurospora NADP-specific glutamate dehydrogenase that was treated with iodoacetate, iodoacetamide, or N-ethylmaleimide to block the thiol groups was cleaved with cyanogen bromide. Of the expected 10 peptides, based on a methionine content of 9 residues, 8 were obtained in pure form and 2 were handled as a mixture. The fragments ranged in size from 9 to 109 residues. In addition, there were isol...
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Three enzymes, (a) nicotinamide adenine diphosphate-dependent glutamic dehydrogenase (NAD enzyme), (b) nictoinamide adenine triphosphate-dependent glutamic dehydrogenase (NADP enzyme), and (c) nicotinamide-adenine dinucleotidase (NADase), were measured in separate extracts of Neurospora crassa grown in Vogel's medium N and medium N + glutamate. Specific activities and total units per culture of...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 252 22 شماره
صفحات -
تاریخ انتشار 1977